Synthetic Culture Media, e.g. KMA
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Medium in which all components are defined
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Most used in experiments where interaction between unknown media
components might affect experimental results : for example in studies
of pH tolerance
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Also used in growing out mating mixes that include donor strains
which have specific vitamin and amino-acid requirements
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Composition: Glycerol, 5.0 g; K2SO4,
0.13 g; K2HPO4, 0.10; Na glutamate, 0.22 g;
MgSO4.7H2O, 0.08 g; CaCl2.2H20,
0.143 g; FeEDTA, 0.04 g; Vitamin solution, 1. 0 mL; Micronutrient
soln, 1.0 mL
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