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Subculturing Organisms on Agar Plates

Colony

  • Aim is to progressively dilute out the culture until you have isolated colonies

  • Colonies in this region should be uniform in size, shape and color with no evidence of comtaminants

NOTE: The air around us, our hands, etc., all contain organisms. To subculture we need all materials to be sterile, and must work in a clean room or laminar flow hood

 


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