Subculturing Organisms on Agar Plates

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Aim is to progressively dilute out the culture until you have
isolated colonies
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Colonies in this region should be uniform in size, shape and color
with no evidence of comtaminants
NOTE: The air around us, our hands, etc., all contain organisms. To
subculture we need all materials to be sterile, and must work in a clean
room or laminar flow hood
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